Synthesis of Phosphatidylserine by Escherichia coli

Phosphatidylserine Synthetase Mutants of Escherichia coli

Mutants of Escherichia coli K-12 defective in CDP-diglyceride:L-serine phosphatidyltransferase (phosphatidylserine synthetase) can be isolated by a rapid autoradiographic screening assay described previously (Raetz, C. R. H. (1975) Proc. N&l. Acad. Sci. U. S. A. 72, 2274-2278). Four organisms of this kind have now been characterized. The gene (designated pss) which is altered in these mutants i...

Partial purification and properties of phosphatidylserine synthetase from Escherichia coli.

CDP-diglyceride: L-serine phosphatidyltransferase (phosphatidylserine synthetase) of Escherichia coli is tightly associated with ribosomes in crude cell-free extracts. The synthetase has now been separated from ribosomes by extraction with solutions containing 5 M NaCl and has been purified loo-fold. The partially purified enzyme is devoid of contaminating hydrolytic activities and nearly free ...

Purification and properties of phosphatidylserine decarboxylase from Escherichia coli.

Phosphatidylserine decarboxylase, a membrane-bound enzyme of Escherichia coli, catalyzes the final step in the biosynthesis of phosphatidylethanolamine, the principal phospholipid of that organism. The enzyme has now been extracted from the membranes with the nonionic detergent Triton X-100 and has been purified about 3600-fold to near homogeneity. The detergent was present throughout the purif...

Case Report of Meningoencephalitis in Calves Caused by Escherichia coli

Synthesis of Pyridoxine by a Pyridoxal Auxotroph of Escherichia coli.

Dempsey, Walter B. (University of Florida, Gainesville). Synthesis of pyridoxine by a pyridoxal auxotroph of Escherichia coli. J. Bacteriol. 92:333-337. 1966.-A pyridoxal auxotroph of Escherichia coli B produced pyridoxol and pyridoxol 5'-phosphate during starvation for pyridoxal. The identification of these compounds was made both by bioassay and by ion-exchange chromatography. Pyridoxol 5'-ph...